Coding

Part:BBa_K3747104

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase C (napC) from Pseudomonas stutzeri JM300

This gene is part of the nap operon from P. stutzeri JM300. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapC is a tetra-heme transmembrane protein and is also considered an essential component of the electron transfer chain to NapA in organisms in which the gene is found [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 332
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] LC, P., & JA, C. (1999). Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12. The Biochemical Journal, 344 Pt 1(Pt 1), 69–76. https://doi.org/10.1042/0264-6021:3440069

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Categories
Parameters
None